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ISO 16212:2017
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Quick understanding of the test
ISO 16212:2017 - Enumeration of yeast and mold in cosmetic samples
Application
- Plate Count Method:
- The product sample is diluted in enrichment broth.
- The diluted test product is inoculated onto a specified agar medium and incubated.
- After incubation, the CFU is counted to quantify yeast and mold.
- Membrane Filtration:
- The product sample is filtered using a suitable membrane filter.
- The filter membrane is transferred onto a specified agar medium and incubated.
- After incubation, the colony-forming units are assessed for contamination levels.
- Detects fungal contamination in products and quantifies the level of contamination.
- Ensures products meet stringent microbiological safety standards.
Turnaround Time
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Abstract
ISO 16212 standard test method lays down the guideline for the enumeration of yeast and molds on selective agar medium after aerobic incubation. However, before starting the microbiological analysis, it is important to ensure that each product sample is neutralized and checked for its antifungal activity. The antifungal/fungicidal activity of product samples can significantly impact the results of microbial risk assessment.
ISO 16212 Test Method
Plate count method
- Initial suspension of the test product is prepared by diluting 1 g or 1 ml test product into an enrichment broth.
- Initial suspension or dilution of the test product is inoculated on a specified agar medium.
- Inoculated petri plates are subjected to aerobic incubation at 25 ยฐC ยฑ 2.5 ยฐC for 3-5 days.
- After incubation, colony-forming units (CFU) are used as a measure to determine the number of yeast and mold in the product sample.
Membrane filtration
- This method involves a suitable amount of product sample to be passed through a membrane filter using a filtration apparatus.
- After filtration, the membrane filter is transferred onto the surface of the specified agar medium and incubated at 25 ยฐC ยฑ 2.5 ยฐC for 3 – 5 days.
Importance of ISO 16212 Test
High levels of contamination of yeast and mold in cosmetic samples is concerning as it can lead to skin irritations and other serious health issues. Hence, assessing microbiological quality of cosmetic products is a pivotal practice to prevent microbial contamination and ensure safe product development for end users.
To address this concern, ISO 16212 standard outlines a quantitative test method to determine the presence of any yeast or mold contamination within the given cosmetic product. ISO 16212 testing helps to ensure that tested cosmetic samples meet the highest standards of safety.
Conclusion
At MIS, we offer cosmetic testing services as per various industry standards such as USP, ISO, and PCPC (formerly CFTA).
Our microbiology testing services for cosmetic products include preservative efficacy testing, and enumeration tests for semi finished and finished formulations.
Our state-of-the-art microbiological testing facilities are equipped with cutting-edge technology. Our team consists of industry experts who have hands-on experiences in setting up and running testing protocols.
If you need any assistance with ISO 16212 test i.e. pricing, turnaround time, and expert
Frequently Asked Questions
DR. Martinoz Scholtz
ISO 16212 is a standardized procedure used for the detection of yeast and mold in cosmetic products. The objective of ISO 16212 test is to ensure the microbiological risk assessment of cosmetic products to ensure public safety.
ISO 16212 is applicable to cream, emulsions, and other similar products.
ISO 16212 test can take 4-5 weeks to complete.
At Microbe Investigations Switzerland, we test for ISO 16212 using the following microbial strains: Candida albicans (MTCC 3017) and Aspergillus niger (ATCC-16888). Additional strains can be added on customer request.
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