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EN 17422

Quantitative Surface Test for the Evaluation of Teat Disinfectants used in the Veterinary Area

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EN 17422:2022 Quantitative surface test method for the evaluation of teat disinfectants used in the veterinary area (phase 2 step 2)

EN 17422 is designed to identify the bactericidal activity of teat disinfectants used in veterinary settings. EN 17422 is a quantitative carrier test (phase 2, step 2) that uses synthetic test surfaces along with other conditions to replicate real-world product usage scenarios within a controlled laboratory setting.

EN 17422 Test Conditions & Requirement

Test Conditions

Bactericidal activity on synthetic skin without mechanical action

Test organism

Escherichia coli (ATCC 8739 and Staphylococcus aureus (ATCC 6538P)

Test temperature 

30 °C ± 1 °C

Minimum contact time

Pre-milking teat disinfectants 

30 s ± 5 s

Post-milking teat disinfectants 

1 min ± 5 s

Maximum contact time

Pre-milking teat disinfectants 

3 min ± 10 s

Post-milking teat disinfectants 

5 min ± 10 s

Other contact times may be selected at intervals of 30 s for contact times up to 1 min and at intervals of 1 min for contact times > 1 min

Interfering substance

Post-milking teat disinfectants 

Pre-milking teat disinfectants

10,0 g/l milk powder

3,0 g/l bovine albumin

EN 17422 Test Method

  • Bacterial test suspension with interfering substance is inoculated onto a synthetic skin test surface and held at 30 °C for a specific period of conditioning.
  • Following the conditioning time, inoculated test surface is immersed in the disinfectant samples / dilutions of the product for a specific contact time.
  • At the end of that contact time, test surface is transferred into the neutralizer.
  • The bacteria are removed from the test surface by ultrasound technique. Bacteria recovered in neutralizing solution are enumerated by plate count method.
  • The number of surviving bacteria recovered from the treated surface is compared with the number of bacteria observed in the control test. (In control test, surface is exposed to water instead of the disinfectant product)
  • As per EN 17422 passing criteria, the test product must demonstrate at least a 4 decimal log (lg) (post-milking disinfectant) or 3 decimal log (lg) (pre-milking disinfectant) reduction when compared with the control test.

Importance of EN 17422 Test

In veterinary settings, the practice teat disinfection before milking and after milking is a vital practice to reduce the number of pathogens causing mastitis. Mastitis is an inflammation of the mammary gland and is predominantly caused by bacterial pathogens and occasionally mycotic or algal microbes. Mastitis infection is a a common concern among dairy animals which results in substantial economic losses within the dairy sector.

EN 17422 test is an important and widely accepted industry standard guideline that helps to verify theclaims of disinfectant products specifically formulated for teat disinfection in veterinary applications.

At MIS, we are adept at providing comprehensive EN 17422 testing services tailored to the unique product needs of our esteemed clients.

We have built a robust testing system that prioritizes quality and precision at every stage of microbial analysis. Our testing procedures are meticulously designed to align with industry benchmarks and standards.

Beyond our proficiency in veterinary disinfectants,we also offer EN 1276 testing for disinfectants intended for use in food, industrial, domestic and institutional areas.

To request a quote for EN 17422 testing, get in touch with our experts now.


EN 17422 test is used to evaluate the microbicidal effectiveness of teat disinfectants used in veterinary areas. EN 17422 specifies phase 2, step 2 method that uses microbially challenged synthetic skin test surface to determine the effectiveness of the teat disinfectant product.

EN 17422 test is applicable for teat disinfectants used in veterinary areas.

EN 17422 test takes 3-4 weeks to complete.

At MIS, we test for EN 17422 using the following microbial strains: Escherichia coli (ATCC 8739) and Staphylococcus aureus (ATCC 6538P).

Contact us for more information

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