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BS EN 14204:2012
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Evaluate Fungicidal or Yeasticidal activity of Chemical disinfectants and Antiseptics
Quick understanding of the test
BS EN 14204:2012 - Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants and antiseptics used in the veterinary area (phase 2, step 1 test)
EN 14204 is phase 2 step 1 test that evaluates the mycobactericidal activity of chemical disinfectants and antiseptics used in the veterinary area.
Application
- Mycobacterium avium
- The test product is mixed with the test suspension and text mixture is maintained for specific contact times.
- A portion of the mixture is neutralized, plated, and incubated to determine viable microorganisms.
- The reduction in viable microorganisms is then quantified and compared with control samples.
- Helps manufacturers meet regulatory standards for product approval and market entry in the veterinary industry.
- Offers insights into the formulation effectiveness, guiding improvements for enhanced mycobactericidal activity.
Turnaround Time
Passing criteria
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Abstract
EN 14204 is a quantitative suspension test method designed to evaluate the mycobactericidal efficacy of chemical disinfectants and antiseptics specifically used in the veterinary sector.
Mycobacteria possess a waxy, lipid-rich cell wall that makes them highly resistant to many disinfectants, necessitating rigorous validation of product efficacy through standards like EN 14204. This Phase 2, Step 1 test method, simulates practical application conditions by including interfering substances and varied contact times during the test, thereby providing a reliable approach to assess the efficacy of the product against Mycobacteria.
Scope of application
The test is applicable to disinfectant products used in the disinfection of veterinary settings like:
- Animal husbandry
- Breeding
- Production
- Transport
- and disposal of all animals (excluding when in food chain following death and entry to the processing industry)
EN 14204 Test Conditions & Requirements
EN 14204 Test Method
- Test product is added to the suspension of test microorganisms and interfering substances.
- Mixture is allowed to interact for specific contact times and temperature conditions.
- At the end of contact time, a portion of the test mixture is immediately neutralized to halt the reaction between test product and reference micrograinisms.
- The test microorganism recovered in the neutralizing solution is further plated and incubated to determine the number of viable microorganisms.
- After incubation, reduction in viable microorganisms is calculated.
- As a passing criteria, EN 14204 specifies that a test product must achieve at least 4 log reduction value against mandatory test microorganisms.
Importance of EN 14204 Test
Resistance of mycobacterial strains to available disinfectants presents a major challenge to achieve infection prevention in veterinary settings. Owing to their complex cell wall structure, mycobacterial strains are hard to kill. Disinfectant product evaluation is an important aspect to understand product efficacy and scope of its improved performance before practical application.
Disinfectants claiming mycobactericidal activity should be tested using methods which are in compliance with international standards. EN 14204 is one of the widely accepted and used standards for evaluation of mycobactericidal activity of chemical disinfectants and antiseptics used in veterinary areas.
Conclusion
MIS offers a comprehensive range of antimicrobial efficacy tests for disinfectants used in veterinary settings, areas of human medicine, and domestic applications.
With extensive experience of microbiological screening and examinations of raw formulation and finished products, we provide customized solutions that are well adhered to international standards.
We make use of the modern microbiological techniques and advanced technologies to ensure scientific interpretation of our test results are accurate and reliable.
Also check, EN 14348 is another frequently performed test method for evaluation of mycobactericidal activity of samples.
Looking for EN 14204 services, our microbiology team experts can provide you with all technical guidance and any other related concerns. Contact our experts here.
Frequently Asked Questions
DR. Martinoz Scholtz
EN 14204 is a phase 2 step 1 suspension test for disinfectants intended for use in veterinary areas. This test evaluates mycobactericidal activity of the test sample. For mycobactericidal claim, EN 14204 demands 4 log reduction of respective test microorganisms as a minimum measure of product efficacy.
EN 14204 test is applicable for veterinary chemical disinfectants and antiseptics products labeled with mycobactericidal claims.
EN 14204 test takes 4-5 weeks to complete.
At Microbe Investigations Switzerland, we test for EN 14204 using the following bacterial strains : Mycobacterium avium.
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