EN 1040 disinfectant test : a Sanitization Protocol for public places

In public spaces, proper sanitizing protocols are of great importance in handling and controlling the transmission of communicable diseases in light of the global health situation. Among many standards that govern such protocols, the European Standard EN 1040:2005 stands out critically because it is used to evaluate the bactericidal activity of chemical disinfectants and antiseptics. The following article shows a complete manual when making a sanitation protocol in compliance with CSN EN 1040, developed especially for public spaces such as hospitals, schools, and other communal points.

Understanding CSN EN 1040:2005

Overview of EN 1040

According to the CSN EN 1040:2005, the general standard prescribes a quantitative suspension test for checking the bactericidal activity of a chemical disinfectant. It is run to measure the performance of antiseptics and disinfectants within laboratory conditions regarding their ability to cause lethal damage to bacteria.

Key Requirements of EN 1040

  • Test Organisms: The standard specifies the use of two selected strains of bacteria, Pseudomonas aeruginosa (ATCC 15442) and Staphylococcus aureus (ATCC 6538), for testing bactericidal efficacy.
  • Concentration and Conditions: Disinfectants are tested at a maximum concentration of 80% that reflects practical dilution scenarios and a temperature of 20°C in a controlled environment for a contact time of 5 minutes.
  • Evaluation Criteria:  A disinfectant can be considered to pass a test provided it can garner a log reduction of not less than 5; that is, a 99.999% reduction in viable number of bacteria.

Designing a Sanitization Protocol

Selection of Disinfectants

  • Compliance with EN 1040: Guarantee that the disinfectants are proven effective in accordance with the standards laid down in EN 1040.
  • Spectrum of Activity: Select products with the broadest range of bacteria targeted.

Formulating the Protocol

Pre-Cleaning

  • Surface Removal of dirt: There should be no visible debris and organic matter before disinfection because organic material reduces the activity of disinfectants.
Application Method

  • Dilution: Disinfectant solutions will be made in accordance with the manufacturers’ recommendations and will conform to the requirements of concentrations as per EN 1040 standard.
  • Technique: Apply properly depending on the spacing and type of surface using either by spraying, fogging, or wiping.
Contact Time

  • Adherence to Standards: Ensure that the disinfectant stays wet on the surface for the full contact time required for full bactericidal effect.
Neutralizing the activity of disinfectant

  • The disinfectant should be properly neutralized to inactivate it before the residual live bacteria is enumerated after the appropriate contact time 
Safety and Compliance

  • PPE Use: This sanitation worker should be garbed with the necessary PPE to protect from chemicals.
  • Ventilation: Ensure adequate ventilation during and after application to mitigate inhalation risks.

Post-Application Measures

  • Re-application Frequency: Indicate the frequency with which re-treatment of a surface is required, taking into consideration the intensity of use of location and the location’s risk assessment.
  • Waste Disposal: Ensure that cleaning materials like cloths and mop heads are disposed of in a manner that will not promote cross-contamination.

Implementation Strategy

Training and Education

  • Staff Training: Staff training will be conducted through a program of regular training sessions on proper preparation, application, and precautions for using disinfectants.
  • Public Awareness: Public awareness of measures taken breeds confidence in the public on the assurance of people’s safety, increasing compliance and cooperation.

Monitoring and Evaluation

  • Efficacy Checks: Routine surface tests should reveal the ability of disinfectants to reduce bacterial loads in the public spaces.
  • Protocol Updates: Regularly review and update the sanitization protocols in compliance with the most up-to-date standards and manufacturer protocols.

The design of an effective sanitization protocol compliant with CSN EN 1040 requires meticulous planning with full knowledge of the chemical agents involved and rigorous training in implementation. This way, public health administrators can effectively reduce the risk of disease transmission in such settings and ensure a healthy community. The same will ensure and reinforce the credibility and accountability of such managing entities.

At Microbe Investigations Switzerland (MIS), we specialize in providing top-notch antimicrobial testing services tailored for a diverse range of products including textiles, coatings, disinfectants, and plastics for clients across the globe. Our team of microbiology experts employs both standard and advanced testing methodologies, specifically designed to meet the needs of our customers.

In addition to EN 1040 for evaluating basic bactericidal activity, we are equipped to conduct comprehensive efficacy assessments using other test methods such as EN 1276 and EN 14476 for ensuring rigorous evaluation of surface disinfectants.

For detailed information about our testing services, state-of-the-art facilities, and competitive pricing, please reach out to our expert team today.

Contact us for more information



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