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ASTM E2613
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Quick understanding of the test
ASTM E2613 - Test Method for Determining Fungus-Eliminating Effectiveness of Hygienic Handwash and Handrub Agents Using Fingerpads of Adults
Application
- Candida albicans
- Aspergillus niger
- A known volume of fungal suspension is applied to demarcated fingerpads and allowed to dry.
- The contaminated hands are exposed to the test agent and control for a specified duration.
- Remaining organisms on the fingerpads are eluted and analysed to determine the fungal colony-forming units (CFU).
- Reductions in CFU are compared between the control and test agent.
- Provides reliable data on the efficacy of handwash and handrub agents to eliminate fungal contamination.
- Ensures consistency and reproducibility across different laboratories and studies.
Turnaround Time
It typically takes 14 – 28 days.
Passing criteria
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Abstract
ASTM E2613 provides a test method and essential passing criteria to assess the fungus-eliminating effectiveness of hygienic handwash and handrub formulations. The method simulates fungal contamination by applying fungal suspensions to fingertips. It evaluates the reduction in fungal levels through handwashing or friction techniques in adult volunteers.
ASTM E2613 Test Conditions and Requirements
ASTM E2613 Test Methodology
The steps involved are as follows:-
- Preparation of Subjects: Subjects are prepared to ensure their hands are free from any antimicrobial substances. This includes providing them with non-antimicrobial personal care products during the study period.
- Application of Fungal Suspension: A well-demarcated area on the fingerpads is treated with a small volume of fungal suspension and is allowed to dry, simulating transient fungal contamination likely to occur in real life.
- Exposure to Test Agent: After drying the fungal suspension, expose the contaminated area to a test agent or control solution for a specified time.
- Elution and Counting: The fingerpads were washed after treatment, and fungi remaining in the fingerpads were recovered using an eluent. Viable fungal cells were enumerated using CFU assays.
- Results: The fungal count reduction is calculated as the difference in CFU counts before and after treatment. This provides a quantitative estimation of test product effectiveness.
Importance of ASTM E2613 Test
Fungi are an often-overlooked but significant threat to human health. Unlike bacteria and viruses, many fungal species can survive on human skin and surfaces for extended periods, increasing the risk of infections. While antibacterial and antiviral claims are common, many hygiene products overlook fungi, despite their ability to persist and spread. ASTM E2613 bridges this gap by providing a standardized, real-world method to prove that hygienic handwash and handrub products are effective at removing transient fungi from the skin.
By validating fungus-eliminating performance under ASTM E2613, manufacturers demonstrate that their everyday hand hygiene solutions truly deliver on safety and cleanliness, building trust with regulatory bodies, clients, and end users.
At Microbe Investigations Switzerland (MIS), we deliver precise, reliable ASTM E2613 testing backed by expert microbiologists and state-of-the-art facilities. Whether youโre developing a new hand hygiene product or validating existing formulations, we help you meet global standards for antifungal performance, giving your brand scientific credibility.
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Frequently Asked Questions
DR. Martinoz Scholtz
The ASTM E2613 test is a standardized procedure to assess the efficacy of hygienic handwash and hand rub agents against fungal contamination. It provides a standardized approach to measure and compare the fungus-reducing efficacy of hand hygiene products.
Hygienic handwashes and hand rubs designed to specifically reduce fungal contamination on the skin can be evaluated based on ASTM E2613.
The ASTM E2613 test period is usually between 14 – 28 days.
Candida albicans ,Aspergillus niger.
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