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ASTM E1053
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Quick understanding of the test
ASTM E1053 - Standard to Assess Virucidal Activity of Chemicals Intended for Disinfection of Inanimate and Nonporous environmental surfaces
Application
- Hepatitis A virus
- Feline Calicivirus
- Influenza A Virus
- A viral suspension is spread over an inanimate, nonporous surface. and allowed to dry.
- The test sample is applied as directed and kept for the specified contact time.
- Eluate from the test surface is collected and titrated to measure the reduction in infectivity compared to a control.
Benefits
- ASTM E1053 mimics the conditions of use, such as testing on nonporous surfaces that are commonly found in medical and public environments.
- Provides a standardized and efficient process for evaluating disinfectant efficacy.
Turnaround Time
Passing criteria
To pass the test, product must achieve a 4-log reduction in viral infectivity in comparison to control .
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Abstract
ASTM E1053 is a surface test to determine virucidal activity of chemicals intended for the disinfection of inanimate and nonporous environmental surfaces. Products to be evaluated for virucidal efficacy using ASTM E1053 include liquid, aerosol, or trigger-spray microbicides.
ASTM E1053 test can be performed for most viruses that are grown and multiply on cell cultures. Additionally, this test can be used for other virus cultivation mediums such as embryonated egg culture.
ASTM E1053 Test Conditions & Requirements
- Test microorganisms used – SARS CoV-2, Beta coronavirus (OC43), Human coronavirus (229E), Influenza A Viruses (H1N1 and H3N2), Poliovirus, Adenovirus, Murine Norovirus, Vaccinia virus, Feline Calicivirus
- Test temperature – 22 ยฑ 2 ยฐC
- Contact time – As suggested by manufacturer
- Interfering substance – Fetal bovine serum or organic soil load
ASTM E1053 Test Method
- An aliquot of the virus suspension is placed on the surface of a sterile, nonporous carrier
- A virus suspension is prepared.
- and is allowed to dry under ambient conditions.
- The dried carrier is treated with the test substance at its use-dilution or applied from an aerosol/trigger sprayer as per manufacturerโs instructions.
- The treated carrier is held at the required contact temperature and time. In a parallel control, the test sample is replaced with an equal volume of buffer solution.
- Following the contact time, an equal volume of neutralizer is added, and the viral film is resuspended by scraping.
- If gel filtration is required, the suspension is passed through a prepared gel filtration column.
- Serial 10-fold dilutions of the neutralized sample are prepared in an appropriate diluent.
- Dilutions are inoculated into 24-well plates containing confluent host cell monolayers, with a minimum of four replicate wells per dilution.
- Plates are incubated under conditions suitable for the replication of the test virus.
- Wells are examined for the presence or absence of cytopathic effects, and results are recorded.
- Logโโ reduction is determined by comparing titers from treated carriers with those from plate recovery controls.
Importance of ASTM E1053 Test Method
Viruses can survive on surfaces for hours or even days, contributing to the spread of infectious diseases. Without effective disinfection, these viruses can spread easily, posing serious health risks.
ASTM E1053 provides a standardized way to verify that surface disinfectants are truly virucidal. It ensures that products claiming to inactivate viruses actually do so, protecting public health and helping reduce viral transmission. By providing consistent, reproducible results, it also helps the manufacturers to meet regulatory requirements.
Strengths of ASTM E1053 Test Method
- Simulates real-world conditions by testing dried viral films on hard, nonporous carriers (e.g., glass, stainless steel), unlike suspension tests.
- Can be performed with multiple viruses, including enveloped (e.g., influenza, coronaviruses) and non-enveloped (e.g., norovirus surrogates, adenovirus) pathogens.
- Allows variation in contact time, soil load, and temperature to reflect intended use environments.
- Recognized by EPA, Health Canada, and other agencies for supporting virucidal claims, making it essential for product registration.
Limitations of ASTM E1053 Test Method
- Does not assess efficacy on porous materials (e.g., fabrics, paper) where virus penetration may differ.
- While realistic for surface contamination, it does not replicate wiping actions, cleaning steps, or continuous contamination cycles.
- Does not evaluate residual antiviral activity or performance after repeated contamination/cleaning cycles.
Conclusion
MIS offers a comprehensive range of antimicrobial testing services. For antimicrobial analysis of samples, our experts adhere to standard guidelines and also follow necessary preventive measures to mitigate contamination threats to provide reliable results.
With extensive research experience pertaining to microbiology analysis and experimental designs, we help our clients to develop and launch safe, effective and high-quality products.
Need help with booking your sample for ASTM E1053 or ASTM E1052 tests? Contact our expert here today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM E1053 test is used to determine the virucidal activity of chemicals intended for disinfection of inanimate and nonporous environmental surfaces.
ASTM E1053 test is applicable to liquid, aerosol, or trigger-spray microbicides used for disinfection of inanimate and nonporous environmental surfaces.
ASTM E1053 test takes 3-4 weeks to complete.
At Microbe Investigations Switzerland, we test for ASTM E1053 using the following viral strains : SARS CoV-2, Beta coronavirus (OC43), Human coronavirus (229E), Influenza A Viruses (H1N1 and H3N2), Poliovirus, Adenovirus, Murine Norovirus, Vaccinia virus, Feline Calicivirus.
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