ISO 22718: 2015

Detection of Staphylococcus aureus in Cosmetic products

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Quick understanding of the test

ISO 22718: 2015 - Detection of Staphylococcus aureus in Cosmetic products

ISO 22718 provides a test method for the detection and identification of Staphylococcus aureus in cosmetic products.

Application

Applicable to cosmetics and personal care products.

Mandatory test strains

  • Test samples are inoculated into an enrichment medium and incubated. Subsequently, they are cultured on selective and differential media. 
  • After incubation, plates are examined for colonial morphology of Staphylococcus aureus, , and additional identification tests are performed to confirm its presence.

Turnaround Time

Typically, the test is completed within 1 week.
The presence or absence of Staphylococcus aureus is determined based on growth in enrichment broth, selective medium, and subsequent confirmatory tests.

Passing criteria

Absence of Staphylococcus aureus in the tested sample indicates compliance with the standard.

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Abstract

ISO 22718 is a standard test method for the detection and identification of the specified microorganism Staphylococcus aureus in a given cosmetic sample.

ISO 22718 test method helps to analyze the specific microbial burden on the basis of presence or absence of Staphylococcus aureus in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. 

ISO 22718 Test Method

  • The product sample is initially grown in an enrichment broth to increase the microbial population.
  • For water-miscible products, 1 ml of product sample is dispersed in 9 ml of enrichment broth. For water-immiscible products, 1g of  product sample is premixed with a dispersing agent (e.g. polysorbate 80) and then dispersed in 9 ml of enrichment broth. For filterable products,  filtration is performed before adding in the enrichment broth.
  • The enrichment medium is incubated at 32.5 °C for 24 to 48 hrs and observed, followed by growth on selective and differential medium.
  • The presence or absence of staphylococcus aureus is concluded only at the end of all the observations.
  • After incubation, petri plates are examined for colonial morphology of Staphylococcus aureus. This is followed by other suitable, cultural and biochemical tests to confirm the presence of Staphylococcus aureus.The presence or absence of staphylococcus aureus is concluded only at the end of all the observations. 

 

Importance of ISO 22718 Test

ISO 22718 standard applies to cosmetic products and personal care products. Staphylococcus aureus is one of the most common microorganisms in cosmetics and personal care products. It is a gram-positive microorganism and a potential pathogen that can adversely affect human health. The presence of pathogenic microorganisms in cosmetic products poses a health risk for consumers.

Therefore, microbial analysis of cosmetic products needs to be carried out to safeguard customer health as well as maintain product quality among the customers.

Conclusion

At MIS, we offer a full suite of microbiology cosmetics testing services.

MIS has a team of experts who create entire frameworks and SOPs for microbiological analysis of cosmetic products. We work closely with our clients to fully understand their unique requirements and deliver tailored solutions.

Our focused approach is to maintain accuracy and precision in every step of our testing process.

Additionally, ISO 22717 comes within the scope of our testing services.

For ISO 22718 testing price and turnaround times, contact our experts here.

Frequently Asked Questions

DR. Martinoz Scholtz

MIS Labs
1. What is ISO 22718 test?

ISO 22718 is a standard test method for screening of Staphylococcus aureus in cosmetic products. This method aims to ensure product quality and safety of customers.

2. What are the Products that can be tested with ISO 22718 test?

ISO 22718 test is applicable to cosmetics and personal care products.

3. What is the Turnaround Time for ISO 22718 test?

ISO 22718 test method takes 1 week to complete.

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