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BS EN 1657: 2016
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Quick understanding of the test
BS EN 1657: 2016 - Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics in the veterinary area
Application
- Candida albicans
- Aspergillus brassiliensis
- The product sample is mixed with a test suspension and interfering substance for a set time.
- An aliquot of the mixture is neutralized and incubated to detect viable microorganisms.
- Microbial colonies are counted and compared with control samples to assess efficacy.
- Provides a reliable and consistent framework to evaluate the antimicrobial efficacy of veterinary disinfectants.
- Assists manufacturers in developing and improving products by providing clear benchmarks for fungicidal and yeasticidal activity.
Turnaround Time
Passing criteria
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Abstract
EN 1657 outlines a Phase 2 step 1 quantitative suspension test to evaluate the fungicidal and yeasticidal efficacy of disinfectants used in veterinary settings. As a Phase 2, Step 1 test, it simulates practical use conditions by using interfering substances like bovine albumin, yeast extract, and reconstituted milk to mimic real-world organic soiling. This standard ensures test products’ efficacy in real-world scenarios.
Scope of Products
EN 1657 test is applicable to products used for disinfection purposes in veterinary areas. This may include the following areas/situations –
- Animal husbandry
- Breeding
- Production
- Transport
- and disposal of live animals (excluding postโmortem processing for the food chain)
EN 1657 Test Conditions & Requirements
EN 1657 Test Method
- Product sample is added to test suspension of microorganisms (yeast cells or fungal spores) in a solution of an interfering substance.
- The mixture is allowed to interact for a specific contact time.
- At the end of the contact time, 1 ml of the mixture is further transferred to a neutralizing solution to halt the disinfectant action.
- After neutralization, the final mixture is incubated for 3 days to check for the presence of any viable microorganisms.
- Colonies are enumerated and compared to control samples.
- To pass the EN 1657 test, the product sample must demonstrate at least a 4-log reduction in the number of viable fungal or yeast cells under specified conditions.
Importance of EN 1657 Test
Fungal and yeast infections are a persistent concern in veterinary and agricultural environments. Disinfection is a crucial practice for ensuring hygiene and reducing the risk of disease outbreaks in veterinary fields including hospitals and animal houses.
Only disinfectants which are tested for their efficacy claims under practical conditions should be used for routine cleaning and disinfection. EN 1657 provides a rigorous, standardized framework to evaluate disinfectant efficacy under realistic soiling scenarios. Its clear benchmarks guide manufacturers in optimizing formulations and demonstrate compliance with regulatory requirements, fostering consistent antimicrobial efficacy across laboratories.
Conclusion
At MIS, we offer a comprehensive range of antimicrobial efficacy testing options for disinfectant formulations.
You can also send us your product sample for disinfectant efficacy testing as per EN 1650 protocol.
For questions about EN 1657 test protocol, turnaround time, and price quote, contact our experts here.
While assessing microbiological quality of products, our microbiology experts ensure quality control by keeping constant vigilance over all lab conditions for each and every step.
We aim to provide product performance analysis reports that our clients can rely on.
Frequently Asked Questions
DR. Martinoz Scholtz
EN 1657 test method evaluates fungicidal or yeasticidal activity disinfectant formulations intended for use in veterinary areas.
The test is applicable to products used for disinfection purposes in veterinary settings like husbandry, breeding, production, transport and disposal of live animals (excluding postโmortem processing for the food chain)
EN 1657 test takes 1-2 weeks to complete.
At Microbe Investigations Switzerland, we test for EN 1657 using the following bacterial and fungal strains: Candida albicans (MTCC 3017) and Aspergillus niger (ATCC-16888). Additional strains can be added on request.
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