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ASTM - G29
Test for algal resistance
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ASTM - G 29 - Test for algal resistance
- Blue-green algae Oscillatoria sp.
- Cyanobacteria are propagated under fluorescent lighting in a propagation tank.
- Treated and untreated samples are suspended in jars containing homogenized cyanobacteria.
- Jars are incubated alternately in darkness and light for 12 hours over 14 days, with reinoculation every 3–4 days.
- After incubation, samples are visually inspected for algae growth.
- Provides a feasible economical method to evaluate algal resistance.
- Ensures product performance against algal degradation in outdoor environments.
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Abstract
The ASTM G29 test method is a standardized laboratory procedure for determining the resistance of plastic films and related materials to algal growth under controlled environmental conditions. This test method was originally introduced in the year 1996 and published with several updates in the years 2002, 2016. The current version is the 4th, published in the year 2021.
This method determines the algal resistance by exposing treated and untreated specimens to a suspension of the blue-green alga Oscillatoria sp. under controlled light–dark cycles for approximately 14 days, with periodic reinoculation to maintain consistent algal activity. At the conclusion of the test, specimens are examined visually for algal attachment and proliferation, allowing qualitative comparison of material performance.
Organisms typically used in this method
Blue-green algae Oscillatoria sp. (cyanobacteria)
ASTM G29 Test Method
- Oscillatoria sp, a blue-green alga, is grown on redwood slats in a propagation tank under fluorescent light.
- Both treated and untreated test samples are immersed in jars containing homogenized Oscillatoria cells and kept stationary.
- The jars are incubated under alternating 12-hour light and dark cycles, with fresh algal reinoculation every 2-3 days.
- After 14 days, samples are removed and placed on white filter paper surfaces to examine and determine the degree of algal growth.
Importance of ASTM G 29
Polymeric films in moist environments often face algal or cyanobacterial growth, leading to discoloration, surface fouling, and reduced durability. The ASTM G29 method serves as an accelerated simulation of real-world exposure, allowing for qualitative comparison of different plastic compositions, coatings, or additive systems designed to inhibit biofilm formation. It is particularly valuable in product development and quality assurance for materials intended for prolonged water or humidity exposure.
Strengths of ASTM G29
ASTM – G 29 is a simple and cost-effective test method for the determination of algal resistance of plastic films. This test procedure typically takes 14 days and the results of the test can be easily evaluated by visual inspection.
Conclusion
At Microbe Investigations Switzerland (MIS), we offer ASTM G29 testing to determine the algal resistance of plastic films, helping you ensure product performance under real-world conditions. Our experts follow standard protocols to deliver reliable, traceable results that support compliance and product claims.
To learn more about our ASTM G29 testing services or to get a quote, contact our team today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM G29 test method determines the efficacy of plastic films integrated with anti – algae additives to resist the growth of surface-growing algae
ASTM G29 test is applicable for polymeric films used in man-made water structure environments such as swimming pools, irrigation ditches, water storage tanks, and artificial ponds.
ASTM G29 testing duration is 14 days to 30 days (incubation time of test materials after inoculation) based on the type of sample and material tested.
At Microbe Investigations Switzerland, we test for the ASTM G29 using the Blue-green algae Oscillatoria sp.
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