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ASTM - G 29
Determination of Algal Resistance of Plastic Films
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ASTM - G 29 - Determination of Algal Resistance of Plastic Films
- Blue-green algae Oscillatoria sp.
- Cyanobacteria are propagated under fluorescent lighting in a propagation tank.
- Treated and untreated samples are suspended in jars containing homogenized cyanobacteria.
- Jars are incubated alternately in darkness and light for 12 hours over 14 days, with reinoculation every 3โ4 days.
- After incubation, samples are visually inspected for algae growth.
- Provides a feasible economical method to evaluate algal resistance.
- Ensures product performance against algal degradation in outdoor environments.
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Abstract
ASTM G29 is a standard test method used to assess the susceptibility of plastic films to algal growth. It helps in evaluating the material’s ability to resist the attachment and proliferation of surface-growing algae under controlled conditions.
ASTM G29 provides a controlled laboratory procedure to simulate these real-world conditions and assesses how well antimicrobial-integrated films prevent or limit algae proliferation. This test is essential for manufacturers seeking to validate product claims, compare formulations, and ensure reliable surface protection in environments prone to persistent moisture and algae growth.
Organisms typically used in this method
Blue-green algae Oscillatoria sp. (cyanobacteria)
ASTM G29 Test Method
- Propagation tank – In the test method, the propagation tank is used to provide the optimal conditions for the growth of cyanobacteria (test inoculum). Cyanobacteria are propagated on both treated and untreated polymeric mesh screens under fluorescent lighting.
- Treated and untreated samples are suspended in jars containing homogenized cyanobacteria.
- Jars are further kept for incubation in both darkness and light for 12 hrs.
- Every 3 – 4 days jars are reinoculated with homogenized cyanobacteria.
- After the completion of 14 days of the test, test samples (both treated and untreated) are separated and evaluated for fungal growth.
Importance of ASTM G 29
Algal buildup on plastic films used in water-containing systems can lead to operational issues, reduced aesthetic appeal, and material degradation.
This method is essential for testing plastic films used in aquatic environments like swimming pools, artificial ponds, or irrigation channels, where algae growth can compromise aesthetics and functionality. By identifying how well a plastic resists algal colonization, manufacturers can ensure long-term durability, cleanliness, and performance of plastic linings in real-world conditions.
Strengths of ASTM G29
ASTM – G 29 is a simple and cost-effective test method for the determination of algal resistance of plastic films. This test procedure typically takes 14 days and the results of the test can be easily evaluated by visual inspection.
Conclusion
At Microbe Investigations Switzerland (MIS), we offer ASTM G29 testing to determine the algal resistance of plastic films, helping you ensure product performance under real-world conditions. Our experts follow standard protocols to deliver reliable, traceable results that support compliance and product claims.
To learn more about our ASTM G29 testing services or to get a quote, contact our team today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM G29 test method determines the efficacy of plastic films integrated with anti – algae additives to resist the growth of surface-growing algae
ASTM G29 test is applicable for polymeric films used in man-made water structure environments such as swimming pools, irrigation ditches, water storage tanks, and artificial ponds.
ASTM G29 testing duration is 14 days to 30 days (incubation time of test materials after inoculation) based on the type of sample and material tested.
At Microbe Investigations Switzerland, we test for the ASTM G29 using the Blue-green algae Oscillatoria sp.
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