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ISO 17721 : 2021
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Quick understanding of the test
ISO 17721 : 2021 - Quantitative Test Method for antibacterial activity of ceramic tile surfaces
Application
Mandatory test strains
- Escherichia coli (ATCC 8739)
- Staphylococcus aureus (ATCC 6538P)
Both control and test samples are inoculated with a bacterial suspension.
Samples are incubated for predetermined time periods.
After the incubation period, the samples are neutralized using a neutralizing solution.
The neutralizing solution is then serially diluted followed by plating, and incubation to determine the viable microbial count.
- Ensures antibacterial claims for ceramic tiles are accurate through standardized, reproducible testing.
- Ensures compliance with international antibacterial standards.
Turnaround Time
Passing criteria
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Abstract
ISO 17721 Test Conditions & Requirement
Sample preparation – 3 treated test samples and 6 untreated control samples ( without any antibacterial activity) of size approximately (50 ± 2) mm × (50 ± 2) mm square or as close to this size are used for testing.
Preconditioning – Both test and control samples are sterilized before the preconditioning step. Conditioning beds are made by placing a grade #1 Whatman filter paper of size 150 mm or equivalent onto a sterilized petri plate. Filter paper is wet thoroughly with sterile deionized water, and then test and control samples are placed individually on wet filter paper. These petri plates are further placed in an incubator maintained at 37 ± 2 °C and ≥75 % relative humidity for 18 h – 24 h. During the incubation period, it’s important to ensure that the filter paper remains wet.
ISO 17721 Test Method
- Both the control and test sample are inoculated with the bacterial suspension. A sterilized polyethylene film is then placed on top of the inoculum to ensure a uniform spread of the bacterial suspension across the surface, while preventing any leakage from edges.
- For 0 hr recovery of bacteria, 3 control samples inoculated with bacterial suspension are immediately transferred to SCDLP broth or other suitable neutralizer. Solution is shaken vigorously To ensure a good recovery of the bacteria from the control surface .
- 3 treated and 3 non-treated samples are incubated at 37 °C ± 1 °C and ≥75 % RH for the desired contact time period. The recommended contact times are 30 min, 1 hr, 4hr, 8hr, and 24 hr, respectively.
- After the incubation period, both treated and untreated test specimens are transferred to neutralizing solution and shaken to remove bacteria from film and test surfaces.
- Washout solution with the recovered bacteria is serially diluted in PBS, and then plated and incubated at 37 °C for 24 h – 48 h to determine the remaining viable microbial count.
Note – Other enumeration techniques such as luminescence can also be used to determine the bacterial viable count.
Importance of ISO 17721 Test
Conclusion
Looking for ISO 17721 testing services for ceramic tile surfaces! For additional details such as price quote, estimated turnaround times, or to schedule a consultation, please feel free to reach out to MIS experts.
At MIS, we offer a comprehensive range of antibacterial testing services, catering to diverse product developmental needs across different sectors. By employing cutting – edge technologies, we consistently uphold the highest standards in all services that we offer to our esteemed clients.
To get a quote on ISO 17721 or want to know more about our testing facilities, contact our experts here.
Frequently Asked Questions
DR. Martinoz Scholtz
At MIS, we test for ISO 17721 using the following microbial strains: Escherichia coli (ATCC 8739 and Staphylococcus aureus (ATCC 6538P).
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