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EN 17422:2022
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Quick understanding of the test
EN 17422:2022 - Quantitative surface test method for the evaluation of teat disinfectants used in the veterinary area (phase 2 step 2)
Application
- Escherichia coli
- Staphylococcus aureus
- A bacterial suspension mixed with an interfering substance is applied to synthetic skin test surfaces and held for a specific period of conditioning.
- Afterward, the test surface is immersed in a disinfectant solution for a specified duration, followed by neutralization, plating, and incubation.
- Post incubation, bacteria are enumerated and reduction rate is determined.
- Provides a standardized method to assess the efficacy of teat disinfectants against specific pathogens.
- Ensures products meet international standards, facilitating easier market entry and broader acceptance.
Turnaround Time
Passing criteria
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Abstract
EN 17422 is designed to evaluate the bactericidal activity of disinfectants applied on the teat skin without mechanical action as a pre/post – milking teat disinfectants used in the veterinary areas. EN 17422 is a quantitative carrier based test (phase 2, step 2) that uses synthetic test surfaces along with other conditions to replicate real-world product usage scenarios within a controlled laboratory setting.
EN 17422 Test Conditions & Requirement
Test Conditions | Bactericidal activity on synthetic skin without mechanical action | |
Test organism | Escherichia coli (ATCC 8739 and Staphylococcus aureus (ATCC 6538P) | |
Test temperature | 30 °C ± 1 °C | |
Minimum contact time | Pre-milking teat disinfectants 30 s ± 5 s | Post-milking teat disinfectants 1 min ± 5 s |
Maximum contact time | Pre-milking teat disinfectants 3 min ± 10 s | Post-milking teat disinfectants 5 min ± 10 s |
Other contact times may be selected at intervals of 30 s for contact times up to 1 min and at intervals of 1 min for contact times > 1 min | ||
Interfering substance Post-milking teat disinfectants Pre-milking teat disinfectants | 10,0 g/l milk powder 3,0 g/l bovine albumin | |
EN 17422 Test Method
- Bacterial test suspension with interfering substance is inoculated onto a synthetic skin test surface and held at 30 °C for a specific period of conditioning.
- Following the conditioning time, inoculated test surface is immersed in the disinfectant samples / dilutions of the product for a specific contact time.
- At the end of that contact time, test surface is transferred into the neutralizer.
- The bacteria are removed from the test surface by ultrasound technique. Bacteria recovered in neutralizing solution are enumerated by plate count method.
- The number of surviving bacteria recovered from the treated surface is compared with the number of bacteria observed in the control test. (In control test, surface is exposed to water instead of the disinfectant product)
- As per EN 17422 passing criteria, the test product must demonstrate at least a 4 decimal log (lg) (post-milking disinfectant) or 3 decimal log (lg) (pre-milking disinfectant) reduction when compared with the control test.
Importance of EN 17422 Test
In veterinary settings, teat disinfection before and after milking is a vital practice to reduce the number of pathogens causing mastitis. Mastitis is an inflammation of the mammary gland and is predominantly caused by bacterial pathogens and occasionally mycotic or algal microbes. Mastitis infection is a common concern among dairy animals which results in substantial economic losses within the dairy sector.
Conclusion
At MIS, we are adept at providing comprehensive EN 17422 testing services tailored to the unique product needs of our esteemed clients.
We have built a robust testing system that prioritizes quality and precision at every stage of microbial analysis. Our testing procedures are meticulously designed to align with industry benchmarks and standards.
Beyond our proficiency in veterinary disinfectants,we also offer EN 1276 testing for disinfectants intended for use in food, industrial, domestic and institutional areas.
To request a quote for EN 17422 testing, get in touch with our experts now.
Frequently Asked Questions
DR. Martinoz Scholtz
EN 17422 test is used to evaluate the microbicidal effectiveness of teat disinfectants used in veterinary areas. EN 17422 specifies phase 2, step 2 method that uses microbe preconditioned synthetic skin surface to be tested against the given disinfectant sample to determine the effectiveness of the teat disinfectant product.
EN 17422 test is applicable for disinfectants intended to be used on the teat skin as a pre/post milking disinfectant in the veterinary areas.
EN 17422 test takes 3-4 weeks to complete.
At MIS, we test for EN 17422 using the following microbial strains: Escherichia coli (ATCC 8739) and Staphylococcus aureus (ATCC 6538P).
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