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ASTM E2362: 2022
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Quick understanding of the test
ASTM E2362: 2022 - Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection
Application
- Staphylococcus aureus (ATCC 6538P)
- Pseudomonas aeruginosa (ATCC 15442)
- Mycobacterium bovis
- Trichophyton mentagrophytes
- Carrier surfaces are inoculated with test organisms, dried, and wiped with disinfectant towelettes.
- Residual disinfectant on the carriers is neutralized, and the carriers are transferred to a subculture medium.
- The subculture medium is incubated to assess the presence or absence of surviving microorganisms.
- Ensures disinfecting towelettes meet antimicrobial standards for public safety.
- Assists manufacturers in meeting regulatory requirements for disinfectant efficacy.
Turnaround Time
It typically takes 3-4 weeks.
Passing criteria
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Abstract
ASTM E2362 is a qualitative test that demonstrates the antimicrobial effectiveness of pre-saturated or impregnated towelette for the disinfection of hard surfaces. The efficacy of the product is determined by assessing the presence or absence of microbial growth after treatment.
ASTM E2362 test conditions and requirements
ASTM E2362 Test Method
- Microbial test suspension with the interfering substance is inoculated on carrier surfaces and allowed to dry visibly.
- The carrier with the dried test organism is then wiped with pre-saturated or impregnated towelettes and kept for specific contact times.
- At the end of contact time, carriers are transferred into a neutralising solution to suppress the microbicidal activity of test substances and recover the surviving test organisms.
- If needed, carriers can be further transferred into a second tube containing a neutralizing solution to effectively neutralize the test substance.
- Subculture from a second neutralising tube is added to the broth medium and incubated for specified time durations.
- After the incubation period, the broth medium is observed to measure turbidity. The degree of turbidity in a broth culture is the measure of microbial growth.
- Positive test carriers are examined by inoculating and incubating subcultures on appropriate solid media.
- Identification of test microorganisms is verified through colony morphology and Gram staining, with additional techniques used if needed.
Importance of ASTM E2362 Test
Antimicrobial towelettes are developed to simplify surface disinfection and provide users with a convenient, ready-to-use option. These disposable wipes are pre-saturated with antimicrobial agents that reduce or eliminate microorganisms on hard, nonporous surfaces.
Before they reach the market, however, itโs critical to validate their performance through standardized laboratory testing. ASTM E2362 provides a recognized method for evaluating the antimicrobial effectiveness of pre-saturated or impregnated towelettes under conditions that reflect their intended use. This ensures reliable data on their ability to control microbial contamination and supports claims made by manufacturers.
Conclusion
Microbe Investigations Switzerland can help you with all your ASTM E2362 testing needs.
Our specialist project managers can take complete responsibility of your project with a comprehensive suite of quality testing services aligned with international standards.
We also specialize in antimicrobial efficacy testing of textiles, paints, coatings, and cosmetics.
To get a price quote on ASTM E2362 or other queries related to our antimicrobial testing services, contact our expert today.
Frequently Asked Questions
DR. Martinoz Scholtz
ASTM E2362 is a qualitative method of antimicrobial efficacy testing for pre-saturated or impregnated towelettes. This test determines whether a given sample product can kill the test microorganism under practical conditions for its intended use.
ASTM E2362 is carried out for towelettes impregnated with antimicrobial agents. Such products are utilised for hard surface disinfection.
Turnaround time for the ASTM E2362 test may vary depending upon incubation periods for bacterial and fungal strains. ASTM E2362 test typically takes 3-4 weeks to complete except for mycobacteria, which can have an incubation period between 60 and 90 days.
At Microbe Investigations, we test for ASTM E2362 using the following microbial strains: Staphylococcus aureus (ATCC 6538P), Pseudomonas aeruginosa (ATCC 15442), Mycobacterium bovis, and Trichophyton mentagrophytes.
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