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BS EN 1275: 2005
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Quick understanding of the test
BS EN 1275: 2005 - Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics (phase 1)
EN 1275 is a Phase 1 quantitative suspension test. It is used to evaluate the basic fungicidal or yeasticidal activity of chemical disinfectants and antiseptics.
Application
- Candida albicans ( ATCC 10231)
- Aspergillus niger( ATCC 16404 )
- A product sample is mixed with a fungal suspension and allowed to interact for a specified time.
- Post contact times, the mixture is transferred to a neutralizing solution and incubated for specific times.
- Viable yeast or fungal colonies are enumerated and compared with control samples.
- Ensures consistent testing procedures across different laboratories, providing reliable and comparable results for basic fungicidal and yeasticidal efficacy.
- Validates products for use in diverse environments, including healthcare, domestic, and industrial settings.
Turnaround Time
Results are evaluated by comparing the reduction in fungal or yeast colonies in the test sample to control samples.
Passing criteria
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Abstract
EN 1275 is a European standard developed to assess the basic fungicidal and yeasticidal activity of chemical disinfectants and antiseptics. It outlines a quantitative suspension test method that evaluates whether product can effectively reduce the number of viable yeast and mould organisms under defined laboratory conditions. EN 1275 is Phase 1 suspension test, hence it helps with initial screening of products that are under development. It forms the basis for further testing required for product approval and regulatory compliance.
Scope of products for EN 1275 testing
EN 1275 test standard is applicable to active substances / antifungal biocides and formulations under development intended to be used in various settings such as –
- Food,
- Industrial,
- Domestic,
- Institutional
- Medical and
- Veterinary areas.
EN 1275 Test Conditions & Requirements
Mandatory test microorganisms – Candida albicans (MTCC 3017) and Aspergillus niger (ATCC-16888)
Temperature – 20 ยฑ 1ยฐC
Contact time – 15 mins
Pass criteria – Test must achieve atleast 4 log reduction to pass the EN 1275 Test.
EN 1275 Test Method
- Test product is challenged with microbial suspension.
- The mixture is allowed to interact for a specific period of time.
- After contact time, the test mixture is further transferred to a neutralizing solution to suppress fungicidal or yeasticidal activity.
- After neutralization, mixture is further incubated for 3 days to check if any viable microorganisms are present.
- Yeast or fungal colonies are enumerated and compared to control samples.
Importance of EN 1275 Test
EN 1275 is important because it provides a standardized method for evaluating the basic fungicidal and yeasticidal activity of disinfectants. As a Phase 1 suspension test, EN 1275 does not assess product performance in real-world scenarios, instead it serves as an essential preliminary screening step to verify the productโs efficacy. It provides crucial efficacy data which is required by manufacturers to proceed further with testing under simulated real-world conditions. Furthermore, since this test is conducted during early stages of formulation development, any negative results obtained during testing can be used by the manufacturers to reform/ make essential improvements in the formulation to ensure better efficacy.
Conclusion
At MIS, our EN 1275 testing services are perfectly curated and executed as per customer requirements. With the help of latest technology and highly qualified professionals, we are able to provide time bound, reliable, and accurate testing services to our clients.
Our antifungal/yeasticidal disinfectant testing portfolio also includes EN 13624 and EN 1650 testing.
You can contact us here in case of any query regarding EN 1275 test or any other relevant method. Our microbiology experts are happy to assist you.
Frequently Asked Questions
DR. Martinoz Scholtz
EN 1275 test is a suspension test (phase 1) which helps to determine the basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics.
EN 1275 test is performed for antiseptics and disinfectant products intended for use in agri-food, industrial, domestic, institutional, medical and veterinary fields.
EN 1275 test takes 3-4 weeks to complete.
At Microbe Investigations Switzerland, we test for EN 1275 using the following strains Candida albicans (MTCC 3017) and Aspergillus niger (ATCC-16888).
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