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AATCC 147
Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method
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AATCC 147 – Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method
AATCC 147 is also known as the Parallel Streak Method. The Parallel Streak Method serves as a relatively quick and easy qualitative method to determine the antibacterial activity of diffusible antimicrobial agents on treated textile materials. However, it is important to note that this test method is not a suitable test for materials that tend to encapsulate and prevent the diffusion of the antibacterial agent or those that contain antibacterial-neutralizing substances.
Test Products
The test method applies to all textile products regardless of the type of antibacterial agents. It includes –
● Clothes
● Waddings
● Threads
● Bedclothes
● Home furnishings
● Other porous materials
Test Organisms
AATCC 147 Test Method
Preparation
- Molten nutrient agar medium is poured into Petri dishes, and allowed to solidify.
Inoculation
- Using a sterile inoculating loop, five parallel streaks of the bacterial suspension are made across the agar surface.
- The treated test specimen (25 x 50 mm) is placed transversely across the bacterial streaks, ensuring proper contact with the agar surface. Meanwhile, a parallel experiment is performed with control (untreated) samples.
Incubation
- The inoculated plates are incubated with the test specimens at 37 ± 2°C for 18-24 hours.
Evaluation
- After incubation, Petri plates are examined for interrupted bacterial growth along the streaks and clear zone of inhibition that occurs due to the presence of the antibacterial agents.
Importance of AATCC 147 standard
AATCC 147 is a valuable test for manufacturers as it allows for quick and accurate determination of the bacteriostatic activity of their products. It also enables manufacturers to screen and validate the performance of antibacterial treatments thus ensuring that their products meet the necessary health and safety standards. Companies can assure consumers of the efficacy of their antibacterial fabrics by performing a thorough AATCC 147 test. This enhances the reliability and marketability of the product across various sectors where hygiene and resistance to microbes are crucial.
Benefits of the test
The test offers several benefits to manufacturers:
- Regulatory compliance –Testing helps meet industry standards and safety regulations.
- Product credibility – Testing validates antimicrobial claims, building consumer trust.
- Quality assurance – Testing ensures overall product quality and reliability.
- Cost-effective – The test is a cost-effective procedure for manufacturers due to its simplicity and rapid execution.
- Competitive edge – Testing helps differentiate products in a crowded market, appealing to health-conscious consumers.
- Innovation support – Testing provides data for developing advanced antimicrobial textiles.
At Microbe Investigations Switzerland, we provide AATCC 147 testing to accurately determine the antimicrobial activity of your textile products. Our specialized testing ensures that your textiles meet the highest standards for antimicrobial efficacy, safeguarding consumer health and product quality. We offer both qualitative (AATCC-147) and quantitative methods (AATCC 100, JIS L 1902 & ISO 20473) for antibacterial testing of textiles.
Looking for fast and accurate antimicrobial testing? Consult our experts today for a quote!
FAQs
AATCC 147, also known as Parallel Streak Method is a qualitative test method specifying the bacteriostatic activity of diffusible antimicrobial agents on textile materials.
The standard applies to all textile products (such as cloth, wadding, thread, bedclothes, and home furnishings) and other porous materials.
It takes 3-4 days to complete this test.
At Microbe Investigations Switzerland, we perform this test using the following bacteria: Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538P), Enterococcus hirae (ATCC 10541), Klebsiella pneumoniae (ATCC 4352), MRSA (ATCC 33591), Salmonella enterica (ATCC 10708), Candida albicans (MTCC 3017). Additional strains can be added on request.
The test detects bacteriostatic activity of textile materials. Specimens of the test material and untreated controls are placed in contact with nutrient agar previously streaked with a test bacterium. After incubation, a clear area of interrupted growth along the sides and underneath the test material indicates antibacterial activity.
Test specimens are cut into rectangular pieces with dimensions of 25 x 50 mm as recommended.
After incubation, the plates are examined for interruption of bacterial growth along the streaks beneath the specimen and a clear zone of inhibition beyond its edge. The width of the inhibition zone is calculated and reported.
The test is a quick and easily executed qualitative method for determining antibacterial activity. It provides evidence of antibacterial activity against both Gram-positive and Gram-negative bacteria which makes it useful for routine quality control and screening tests.
No, this test is specifically designed for textile materials.
This test provides a standard method to demonstrate the presence and effectiveness of antibacterial agents in textiles thus supporting product development and quality assurance.
The test method is unsuitable for materials that can prevent the diffusion of the antibacterial agent or those containing antibacterial-neutralizing substances. It also does not provide a quantitative evaluation of antibacterial activity.
Regular testing is recommended to ensure consistent antibacterial performance of the textiles, especially after multiple washings and under various conditions.
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ASTME2149 | AATCC-100 | JISZ2801 | JISL1902 | EN 1276 | ISO 22196 | ISO 20743 | ISO 846 | ASTM E2180 | AATCC 147 | ASTM D7907-14 | ASTM E3031 | ASTM G29 | EN 16615 | EN 13697 | PAS 2424 | EN 1040 | EN 14349 | EN 13727 | EN 14561 | ASTM G22 | ASTM E1153 | ASTM D2574 | AATCC 174 | EN 14563 | EN 13704 | EN 1499 | EN 1500 | EN 14347 | EN 14348 | EN 1656 | EN 16437 | EN 12791 | ASTM E1174 | ASTM E2362
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